Regulatory

Part:BBa_J100231:Design

Designed by: Kelly Friers   Group: Campbell M Lab   (2015-09-10)


tolC Constitutive Promoter in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We considered modifying the -10 sequence to match the consensus sequence of E. coli because of the strongly negative score from the position weight matrix that our sequence scored. We decided to keep our original sequence and not modify it to test the effectiveness of the original promoter. There was no position weight matrix available to compare with the -35 sequence.


Source

The part comes from a promoter for the tolC gene that encodes a major outer membrane protein in E. Coli.

References